KMID : 0043320070300060761
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Archives of Pharmacal Research 2007 Volume.30 No. 6 p.761 ~ p.769
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Spleen Tyrosine Kinase Participates in Src-Mediated Migration and Proliferation by PDGF-BB in Rat Aortic Smooth Muscle Cells
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Lee Hwan-Myung
Kim Hyo-Jin Park Hyo-Jun Won Kyung-Jong Kim Jung-Hwan Shin Hwa-Sup Park Pyo-Jam Lee Kyung-Yung Park Seung-Hwa Kim Hyun-Jun Lee Chang-Kwon Kim Bo-Kyung
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Abstract
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Tyrosine kinases, Src and spleen tyrosine kinase (Syk), play crucial roles in cell responses to platelet-derived growth factor (PDGF) and may have their functional interactions. In this study, we focused on investigating the roles of Syk in the regulation of Src signaling in PDGF-mediated vascular cell responses. Migration, proliferation, and activity of kinases were determined in rat aortic smooth muscle cells (RASMCs). PDGF-BB (10 ng/mL) induced the migration and proliferation of RASMCs, which were significantly inhibited by PP2 (10 ¥ìM) and piceatannol (30 ¥ìM), inhibitors of Src and Syk, respectively. The phosphorylation of Syk induced by PDGFBB was abolished by PP2. PDGF-BB increased the co-association of the PDGF¥â-receptor and the kinases, Src or Syk, and its maximal binding to Src was achieved in a shorter time than that to Syk. PDGF-BB stimulated the phosphorylation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK) 1/2, which was inhibited by PP2 and piceatannol. PDGF-BB-induced proliferation and migration were inhibited by SB203580 (30 ¥ìM) and PD98059 (30 ¥ìM), inhibitors of p38 MAPK and ERK1/2, respectively. These results imply that Syk is regulated by Src kinase, which participates in migration and proliferation in response to PDGF-BB in RASMCs.
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KEYWORD
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Src, Syk, Proliferation, Migration, RASMC
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